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Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique
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Developing conversed microsatellite markers and their implications in evolutionary analysis of the Bemisia tabaci
Hua-Ling Wang1, Jiao Yang1, Laura M Boykin2
1Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
Scientific Reports
|September 16, 2014
Summary
New microsatellite markers were developed from transcriptome data for the Bemisia tabaci complex. These markers are conserved and effective for population genetics and distinguishing cryptic species.
Area of Science:
- Genomics
- Population Genetics
- Entomology
Background:
- Population genetic studies of the Bemisia tabaci complex are hindered by a scarcity of conserved molecular markers.
- Understanding genetic diversity is crucial for managing this significant agricultural pest.
Purpose of the Study:
- To identify and validate novel microsatellite markers from transcriptome sequences for the Bemisia tabaci complex.
- To assess the utility of these markers for analyzing genetic relationships among cryptic species.
Main Methods:
- New polynucleotide microsatellites were identified from transcriptome sequences of three Bemisia tabaci cryptic species.
- Cross-species transferability of 57 microsatellites was experimentally validated.
- Thirteen validated microsatellites were used to infer phylogenetic relationships among six cryptic species.
Main Results:
- Hundreds of new microsatellite markers were identified.
- The developed microsatellite markers demonstrated high conservation and inter-taxon transferability.
- The inferred phylogeny using microsatellites was consistent with mitochondrial COI sequence data, confirming their ability to distinguish species.
Conclusions:
- Transcriptome data provides a rapid and cost-effective method for developing microsatellite markers.
- These novel microsatellite markers are valuable tools for population genetics and evolutionary studies of the Bemisia tabaci complex.
- The markers can effectively differentiate cryptic species within the complex.
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