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Collagen-based silver nanoparticles for biological applications: synthesis and characterization
Vinicius S Cardoso1,2, Patrick V Quelemes3, Adriany Amorin4
1Research Center in Biodiversity and Biotechnology (Biotec), Campus Parnaíba, Federal University of Piauí, Av São Sebastian 2819, 64202-020, Parnaíba, Piauí, Brazil. vscfisio@ufpi.edu.br.
Journal of Nanobiotechnology
|September 17, 2014
Summary
This study synthesized collagen-stabilized silver nanoparticles (AgNPcol) for biomedical use. The optimized AgNPcol formulation demonstrated antibacterial properties and cell viability, making it a promising biomaterial.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Materials Chemistry
Background:
- Type I collagen is a natural polymer with tissue regeneration applications.
- Silver nanoparticles (AgNPs) are versatile nanotechnology materials with medical utility.
- This study synthesized AgNPs stabilized with type I collagen (AgNPcol) for biological applications.
Purpose of the Study:
- To synthesize and characterize silver nanoparticles stabilized with type I collagen (AgNPcol).
- To evaluate the physicochemical properties, antibacterial activity, and cell viability of AgNPcol formulations.
- To identify an optimal AgNPcol formulation for potential biomedical applications.
Main Methods:
- Synthesis of three AgNPcol formulations with varying molar ratios.
- Physicochemical characterization using UV-Vis spectroscopy, DLS, FTIR, AAS, TEM, and XRD.
- In vitro antibacterial activity assays against Staphylococcus aureus and Escherichia coli.
- Cell viability assays on OSCC cells.
Main Results:
- All AgNPcol formulations exhibited spherical shapes and positive zeta potential.
- The 1:6 molar ratio AgNPcol showed superior characteristics: smaller hydrodynamic diameter (64.34 nm), lower PDI (0.40), and higher silver reduction efficiency.
- AgNPcol demonstrated significant antimicrobial activity against S. aureus and E. coli with no observed toxicity to OSCC cells.
Conclusions:
- The synthesized AgNPcol particles possess favorable characteristics for biomedical applications.
- Optimal formulation (1:6 ratio) displayed spherical morphology, suitable size (64.34–81.76 nm), positive zeta potential, and effective antibacterial activity.
- The non-toxic nature of AgNPcol to tested cells (OSCC) supports its potential as a safe and effective biomaterial.
