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Updated: Apr 23, 2026
10:54
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
Published on: February 23, 2016
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Chemical reaction-induced multi-molecular polarization (CRIMP)
Y Lee1, N M Zacharias, D Piwnica-Worms
1Hanyang University, Department of Applied Chemistry, Ansan, 426-791, Korea.
Chemical Communications (Cambridge, England)
|September 17, 2014
Summary
We developed a new hyperpolarization technique, Chemical Reaction-Induced Multi-molecular Polarization (CRIMP), to study multiple biological processes like metabolism and pH in vivo simultaneously. This method uses pyruvic acid to create four distinct imaging agents.
Area of Science:
- Metabolic imaging
- Biophysical chemistry
- Medical physics
Background:
- Hyperpolarization techniques enhance low-sensitivity nuclear spins for improved MRI.
- Current methods often target single metabolic pathways.
- Simultaneous in vivo monitoring of multiple cellular processes remains a challenge.
Purpose of the Study:
- To introduce a novel hyperpolarization method, Chemical Reaction-Induced Multi-molecular Polarization (CRIMP).
- To enable simultaneous in vivo imaging of diverse metabolic pathways and pH.
- To generate multiple hyperpolarized agents from a single precursor.
Main Methods:
- Development of the CRIMP technique utilizing non-enzymatic decarboxylation.
- Synthesis of four distinct hyperpolarized imaging agents from hyperpolarized 1,2-(13)C pyruvic acid.
- Application of the method for simultaneous in vivo studies.
Main Results:
- Successful generation of four unique hyperpolarized agents.
- Demonstration of CRIMP's potential for simultaneous in vivo metabolic and pH mapping.
- Potential application to study glycolysis, TCA cycle, fatty acid synthesis, and pH.
Conclusions:
- CRIMP offers a versatile platform for multi-parametric in vivo metabolic imaging.
- This technique advances the simultaneous study of complex biological processes.
- CRIMP expands the utility of hyperpolarized agents derived from pyruvic acid.
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