Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioavailability Study Design: Healthy Subjects Versus Patients01:15

Bioavailability Study Design: Healthy Subjects Versus Patients

240
Bioavailability studies are essential for evaluating a drug's therapeutic efficacy and understanding its absorption patterns under various physiological conditions. Conducting such studies on target patient populations provides more relevant data by simulating real-world disease states. However, practical challenges often necessitate the use of young, healthy adult volunteers as study subjects.Patients may exhibit altered drug absorption patterns due to the effects of the disease itself,...
240
Bioavailability Study Design: Single Versus Multiple Dose Studies01:11

Bioavailability Study Design: Single Versus Multiple Dose Studies

375
Bioavailability studies are essential for understanding how a drug is absorbed, distributed, metabolized, and excreted in the body. These studies assess the extent and rate at which the active pharmaceutical agent becomes available at the site of action. The design of bioavailability studies can involve single-dose or multiple-dose regimens, each with distinct advantages and limitations.Single-dose studies are the preferred approach due to their simplicity and reduced drug exposure for...
375
Preclinical Development: Overview01:28

Preclinical Development: Overview

4.7K
Preclinical development consists of a series of tests that ensure the safety and efficacy of a new therapeutic compound before it is tested in humans. There are four main phases to this process. First, safety pharmacology tests are conducted to ensure the drug does not produce any acutely harmful effects. These tests examine parameters such as bronchoconstriction, cardiac dysrhythmias, blood pressure changes, and ataxia. Next, preliminary toxicological testing is performed to determine the...
4.7K
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

828
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
828

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bio-SCOPE: fast biexponential T<sub>1ρ</sub> mapping of the brain using signal-compensated low-rank plus sparse matrix decomposition.

Magnetic resonance in medicine·2019
Same author

Enantioselective Radical Ring-Opening Cyanation of Oxime Esters by Dual Photoredox and Copper Catalysis.

Organic letters·2019
Same author

ACCELERATING MAGNETIC RESONANCE IMAGING VIA DEEP LEARNING.

Proceedings. IEEE International Symposium on Biomedical Imaging·2019
Same author

Technical note: Development and application of KASP assays for rapid screening of 8 genetic defects in Holstein cattle.

Journal of dairy science·2019
Same author

Sesquiterpenes and diterpenes from Euphorbia thymifolia.

Fitoterapia·2019
Same author

Glechomanamides A-C, Germacrane Sesquiterpenoids with an Unusual Δ<sup>8</sup>-7,12-Lactam Moiety from <i>Salvia scapiformis</i> and Their Antiangiogenic Activity.

Journal of natural products·2019

Related Experiment Video

Updated: Apr 23, 2026

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
14:39

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Published on: April 22, 2022

3.5K

Pre-formulation studies of resveratrol.

Keila Robinson1, Charlotta Mock, Dong Liang

  • 1a Department of Pharmaceutical Sciences , Texas Southern University , Houston , TX , USA.

Drug Development and Industrial Pharmacy
|September 17, 2014
PubMed
Summary

Resveratrol exhibits good solubility in solvents like PEG-400 and is stable in acidic conditions. This natural compound shows high plasma protein binding and slower degradation in human plasma compared to rat plasma.

Keywords:
Natural compoundphysiochemical evaluationplasma protein bindingpolyphenolsolubilitystability

More Related Videos

Author Spotlight: Exploring Seaweed's Bioactive Compounds for Sustainable Innovations in Industries
10:18

Author Spotlight: Exploring Seaweed's Bioactive Compounds for Sustainable Innovations in Industries

Published on: November 21, 2023

3.3K
Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds
07:13

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Published on: August 8, 2025

1.8K

Related Experiment Videos

Last Updated: Apr 23, 2026

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
14:39

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Published on: April 22, 2022

3.5K
Author Spotlight: Exploring Seaweed's Bioactive Compounds for Sustainable Innovations in Industries
10:18

Author Spotlight: Exploring Seaweed's Bioactive Compounds for Sustainable Innovations in Industries

Published on: November 21, 2023

3.3K
Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds
07:13

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Published on: August 8, 2025

1.8K

Area of Science:

  • Pharmacology and Drug Development
  • Natural Products Chemistry

Background:

  • Resveratrol, a compound from grapes, has potential anticancer properties.
  • Its clinical use is limited by very low oral bioavailability in humans.

Purpose of the Study:

  • To assess the solubility of resveratrol in various solvents.
  • To determine its pH stability profile.
  • To evaluate plasma protein binding (PPB) and stability in human and rat plasma.

Main Methods:

  • Solubility measured using High-Performance Liquid Chromatography (HPLC).
  • pH stability assessed in USP buffers (pH 2-10) at 37°C.
  • Human PPB determined via ultracentrifugation; plasma stability evaluated at 37°C.

Main Results:

  • Resveratrol solubility varied widely (0.05 mg/mL in water to 374 mg/mL in PEG-400).
  • It remained stable above pH 6, with peak degradation at pH 9.
  • Mean PPB was 98.3%; half-lives in human and rat plasma were 54h and 25h, respectively.

Conclusions:

  • Resveratrol demonstrates enhanced solubility in solvents like PEG-400 and stability in acidic environments.
  • High plasma protein binding and slower degradation in human plasma suggest potential for improved therapeutic strategies.