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Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes
Yan-Jiun Lee1, Yadagiri Kurra, Yanyan Yang
1Department of Chemistry, Texas A&M University, College Station, TX 77843, USA. wliu@chem.tamu.edu.
Abstract:
A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells.

