Quantification of HDL particle concentration by calibrated ion mobility analysis
Patrick M Hutchins1, Graziella E Ronsein1, Jeffrey S Monette1
1Department of Medicine, University of Washington, Seattle, WA;
Clinical Chemistry
|September 17, 2014
Summary
Calibrated ion mobility analysis (calibrated IMA) accurately quantifies HDL particle concentration (HDL-P). This new metric is lower in cerebrovascular disease patients, independent of HDL cholesterol, offering a better measure of HDL cardioprotection.
Area of Science:
- Cardiovascular Research
- Biomarker Discovery
- Analytical Chemistry
Background:
- Current methods for measuring HDL particle concentration (HDL-P) show >5-fold variability.
- Accurate HDL-P measurement is critical for assessing HDL's cardioprotective role in humans.
Purpose of the Study:
- To develop and validate an improved method for quantifying HDL particle concentration.
- To assess the association of HDL-P with cardiovascular disease status.
Main Methods:
- Developed calibrated ion mobility analysis (calibrated IMA) for HDL-P quantification.
- Isolated HDL via ultracentrifugation, used electrospray ionization, and size-based separation.
- Employed a calibration curve with purified proteins to correct for ionization efficiency.
Main Results:
- Calibrated IMA accurately measured HDL-P in gold nanoparticles and reconstituted HDLs.
- Measured HDL-P in control and cerebrovascular disease participants (n=80).
- Found significantly lower HDL-P in cerebrovascular disease patients, independent of HDL cholesterol.
Conclusions:
- Calibrated IMA accurately quantifies HDL particle concentration, aligning with structural models.
- HDL-P is a potential biomarker for cardiovascular disease status.
- This method offers improved assessment of HDL's cardioprotective potential.
