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Updated: Apr 23, 2026

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
Published on: October 11, 2024
JIP60-mediated, jasmonate- and senescence-induced molecular switch in translation toward stress and defense protein
Sachin Rustgi1, Stephan Pollmann2, Frank Buhr3
1Department of Crop and Soil Sciences, School of Molecular Biosciences, and Center for Reproductive Biology, Washington State University, Pullman, WA 99164-6420; rustgi@wsu.edu diter@wsu.edu sreinbot@ujf-grenoble.fr.
Abstract:
Two closely related genes encoding the jasmonate-induced protein 60 (JIP60) were identified in the barley genome. The gene on chromosome arm 4HL encodes the previously identified protein encoded by the cDNA X66376.1. This JIP60 protein is characterized here and shown to consist of two domains: an NH2-terminal domain related to ribosome-inactivating proteins and a COOH-terminal domain, which displays similarity to eukaryotic translation initiation factor 4E (eIF4E). JIP60 undergoes processing in vivo, as a result of which JIP60's COOH-terminal eIF4E domain is released and functions in recruiting a subset of cellular messengers for translation. This effect was observed for both MeJA-treated and naturally senescing plants. Because the JIP60 gene is in close proximity to several quantitative trait loci for both biotic and abiotic stress resistance, our results identify a unique target for future breeding programs.
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