Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lambda cro repressor complex with OR3 operator DNA. 19F nuclear magnetic resonance observations.

W J Metzler1, P Lu

  • 1Department of Chemistry, University of Pennsylvania, Philadelphia 19104.

Journal of Molecular Biology
|January 5, 1989
PubMed
Summary

This study reveals unexpected DNA distortions and environmental asymmetries when lambda cro repressor binds to OR3 DNA operators. These findings challenge current models of protein-DNA interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Efficacy analysis of maintenance therapy with levamisole compared to mycophenolate mofetil after rituximab treatment in children].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2026
Same author

Matrine inhibits the proliferation, migration, invasion, and stemness of thymoma cells by regulating the Wnt3a/Β-catenin pathway.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2026
Same author

Long-Range Transverse-Momentum Correlations and Radial Flow in Pb-Pb Collisions at the LHC.

Physical review letters·2026
Same author

Dosing parameters for grafting human neural stem cells into sites of spinal cord injury.

Experimental neurology·2025
Same author

Multidimensional impacts of oil spills in transit: An overview of trend analysis, remediation strategies, and community resilience.

Journal of environmental management·2025
Same author

Search for Quasiparticle Scattering in the Quark-Gluon Plasma with Jet Splittings in pp and Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2025

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • The lambda cro repressor protein plays a crucial role in bacteriophage lambda DNA regulation.
  • Understanding the precise interactions between repressors and their DNA operator sites is fundamental to gene regulation studies.

Purpose of the Study:

  • To investigate the detailed interaction between lambda cro repressor and its synthetic OR3 DNA operator.
  • To probe DNA structural changes and environmental asymmetries upon protein binding using fluorine NMR spectroscopy.

Main Methods:

  • Synthesis of 17 base-pair OR3 operator DNA with 5-fluorodeoxyuridine substitution at thymidine positions.
  • Nuclear Magnetic Resonance (NMR) spectroscopy, specifically monitoring fluorine chemical shifts in varying D2O concentrations.

Related Experiment Videos

  • Analysis of protein-DNA complex structure in relation to existing structural models.

    • Main Results:

    • Identified an asymmetry in the DNA operator's ends despite the symmetry of the cro repressor dimer and binding site.
    • Observed DNA helix distortion at two specific positions within the operator.
    • Detected DNA environmental changes in the helix's central region, suggesting distortions beyond predicted contact sites.

    Conclusions:

    • The study reveals previously unpredicted DNA structural rearrangements and environmental asymmetries during cro repressor-OR3 binding.
    • Findings indicate that current models may not fully capture the dynamic and complex nature of protein-DNA interactions.
    • Suggests the need for refined models that account for broader DNA distortions and asymmetric interactions.