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Plasmon enhanced fluorescence with aggregated shell-isolated nanoparticles.

Igor O Osorio-Román1, Ariel R Guerrero, Pablo Albella

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Inducing aggregation of shell-isolated nanoparticles (SHINs) with electrolytes significantly enhances their signal amplification in shell-isolated nanoparticle-enhanced fluorescence (SHINEF) experiments. This method can boost the enhancement factor by up to 10-fold.

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Area of Science:

  • Nanotechnology
  • Surface Chemistry
  • Spectroscopy

Background:

  • Shell-isolated nanoparticles (SHINs) offer well-defined localized surface plasmon resonances (LSPRs).
  • The SiO2 coating in SHINs for shell-isolated nanoparticle-enhanced fluorescence (SHINEF) is thicker than the critical distance for metal quenching.
  • SHINs serve as versatile substrates for various optical applications.

Purpose of the Study:

  • To investigate methods for enhancing the signal amplification in SHINEF experiments.
  • To demonstrate that inducing aggregation of SHINs can increase the SHINEF enhancement factor.
  • To explore the effect of electrolyte concentration on SHIN aggregation and SHINEF performance.

Main Methods:

  • Utilizing electrolytes, such as NaCl, to induce aggregation of gold and silver SHINs in solution.
  • Controlling the surface concentration of gold SHINs (Au-SHINs) by spraying techniques.
  • Fabricating samples by spraying Au-SHINs onto layer-by-layer (LbL) and Langmuir-Blodgett (LB) films.
  • Incorporating fluorophores with varying quantum yields into the fabricated samples for SHINEF experiments.

Main Results:

  • Inducing SHIN aggregation with electrolytes leads to a significant increase in the SHINEF enhancement factor (EF).
  • A 10-fold enhancement in EF was achieved by optimizing electrolyte concentrations.
  • The aggregation effect was studied in conjunction with controlling the surface concentration of Au-SHINs via spraying.
  • SHINEF experiments were successfully conducted on LbL and LB films using different fluorophores.

Conclusions:

  • SHIN aggregation is a viable strategy to boost SHINEF signal amplification.
  • Electrolyte-controlled SHIN aggregation offers a tunable approach to enhance SHINEF performance.
  • The findings provide a pathway for optimizing SHINEF-based sensing and analytical techniques.