Glucose biosensing using glassy carbon electrode modified with polyhydroxy-C60, glucose oxidase and ionic-liquid

Area of Science:

• Electrochemistry
• Biotechnology
• Materials Science

Background:

• Direct electrochemistry of enzymes is crucial for biosensor development.
• Functional membranes can enhance enzyme immobilization and electron transfer.
• Polyhydroxyfullerenes offer unique properties for material functionalization.

Purpose of the Study:

• To develop a biosensor for direct glucose detection using glucose oxidase (GOD).
• To investigate the electrochemical properties of an ionic liquid/GOD-Polyhydroxy-C60 functional membrane.
• To evaluate the performance of the modified electrode for glucose sensing.

Main Methods:

• Fabrication of a functional membrane using ionic liquid, GOD, and Polyhydroxy-C60.
• Immobilization of the membrane onto a glassy carbon electrode (GCE).
• Electrochemical characterization using cyclic voltammetry (CV).

Main Results:

• Achieved direct electrochemistry of GOD with a formal potential (E°') of -329 ± 2 mV.
• Determined a heterogeneous electron transfer constant (k(s)) of 1.43 s⁻¹.
• Observed a linear response to glucose from 0.02 to 2.0 mM with a detection limit of 1 μM.

Conclusions:

• The developed ionic liquid/GOD-Polyhydroxy-C60 membrane on GCE enables efficient direct electrochemistry for glucose sensing.
• The biosensor exhibits excellent sensitivity and a low detection limit for glucose.
• This approach holds promise for advanced electrochemical biosensor applications.