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An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
Published on: May 6, 2021
Stem cells from human exfoliated deciduous teeth differentiate into functional hepatocyte-like cells by herbal
1Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1 Sec. 3, Chung-Hsiao E. Rd, Taipei 10608, Taiwan.
Abstract:
Stem cells from human exfoliated deciduous teeth (SHEDs) are mesenchymal stem cells isolated from the exfoliated human deciduous incisor that can differentiate into a many cell types. In this study, we evaluated the effect of liquorice or angelica extracts on the hepatic differentiation potential of SHEDs cells. SHEDs cells cultured in medium containing liquorice extracts were analyzed for 1) changes in cellular morphology, 2) changes in hepatic gene expression, AFP (Alpha-fetoprotein) and ALB (Albumin), and 3) albumin secretion and urea synthesis activity. Our data show that the hepatic differentiation potential of SHEDs cells is enhanced by the presence of liquorice or angelica extracts in the culture medium. Our findings present new therapeutic possibilities for liver damage repair.
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