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Updated: Apr 23, 2026
A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents
Published on: July 16, 2020
Fluorescence imaging of Evans blue extravasation into mouse brain induced by low frequency ultrasound with
Yuanyuan Shen1, Aili Zhang2, Jinxuan Guo3
1Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Shenzhen 518060, China National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, Shenzhen 518060, China.
Abstract:
Blood-Brain Barrier (BBB) can be opened locally, noninvasively and reversibly by low frequency focused ultra-sound (FUS) in the presence of microbubbles. In this study, Evans blue (EB) dye extravasation across BBB was enhanced by 1 MHz FUS at acoustic pressure of 0.35 MPa in the presence of microbubbles at clinically comparable dosage. The spatial distribution of EB extravasation was visualized using fluorescence imaging method. The center region of BBB disruption area showed more enhanced fluorescence signal than the surrounding region in general. However, EB dye deposition was heterogeneous in the center region. The findings in this study indicated potential use of fluorescence imaging to evaluate large molecules delivery across BBB.
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