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Cloning and sequence analysis of cDNA encoding a precursor for rat brain natriuretic peptide
M Kojima1, N Minamino, K Kangawa
1Department of Biochemistry, Miyazaki Medical College, Japan.
Biochemical and Biophysical Research Communications
|March 31, 1989
Summary
Researchers identified rat brain natriuretic peptide (BNP) precursor by constructing a cDNA library. High homology was found between rat and porcine BNP precursors, aiding in understanding BNP family characteristics.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Brain natriuretic peptide (BNP) is a peptide hormone found in the brain and atrium.
- Immunological studies suggested potential structural differences in BNP between rat and porcine species.
Purpose of the Study:
- To identify and characterize the rat brain natriuretic peptide (BNP) precursor.
- To investigate structural similarities and differences between rat and porcine BNP.
Main Methods:
- Construction of a rat atrial cDNA library.
- Screening of the library using porcine BNP cDNA as a probe.
- Sequencing of the cloned cDNA to determine the amino acid sequence of rat BNP precursor.
Main Results:
- Deduced the amino acid sequence of the rat BNP precursor (121 residues).
- Identified a 26-residue putative signal peptide at the N-terminus.
- Observed homology between rat BNP precursor and porcine BNP-32 at the C-terminus.
- Found significant homology in the 3'-untranslated region between rat and porcine BNP precursors.
Conclusions:
- Structural and processing features characteristic of the BNP family were discussed based on sequence comparisons.
- The study provides insights into the molecular characteristics of rat BNP and its relationship to porcine BNP.