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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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DNA Isolation

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DNA Helicases

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Updated: Feb 10, 2026

Identification of Mycobacterium Species by DNA Microarray Chip Method

Identification of Mycobacterium Species by DNA Microarray Chip Method

Published on: June 24, 2025

Microarray-based cell-free DNA analysis improves noninvasive prenatal testing.

Kara Juneau¹, Patrick E Bogard, Stephanie Huang

¹Ariosa Diagnostics Inc., San Jose, Calif., USA.

Fetal Diagnosis and Therapy

|September 18, 2014

Summary

This summary is machine-generated.

Microarray-based noninvasive prenatal testing (NIPT) offers faster and more accurate cell-free DNA analysis compared to next-generation sequencing, improving fetal fraction precision for trisomy detection.

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Area of Science:

Genetics
Molecular Biology
Bioinformatics

Background:

Noninvasive prenatal testing (NIPT) is crucial for detecting fetal chromosomal aneuploidies.
Current NIPT methods, primarily next-generation sequencing (NGS), face limitations in speed and accuracy.
Developing advanced microarray techniques is essential for improving NIPT performance.

Purpose of the Study:

To develop and validate a novel microarray-based method for NIPT.
To compare the diagnostic accuracy and efficiency of the microarray method against NGS.

Main Methods:

Maternal plasma from 878 pregnancies, including 187 trisomy cases, were analyzed.
Digital Analysis of Selected Regions (DANSR™) assays targeted specific chromosomal regions.
Cell-free DNA (cfDNA) was quantified using both microarrays and NGS, analyzed with the Fetal-Fraction Optimized Risk of Trisomy Evaluation (FORTE™) algorithm.

Main Results:

Microarray-based NIPT demonstrated significantly lower assay variability (0.051 vs. 0.099) and faster analysis time (7.5 vs. 56 hours) compared to NGS.
Fetal fraction precision was improved 1.6-fold with microarrays due to increased polymorphic site analysis.
The microarray method achieved 100% accuracy in classifying all trisomy and non-trisomy cases.

Conclusions:

Microarray-based NIPT provides superior cfDNA analysis accuracy and efficiency over NGS.
This method offers a faster and more precise approach for prenatal screening of trisomies.
Microarrays represent a promising advancement in noninvasive prenatal diagnostic technologies.