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High stability of apo-cytochrome c' from thermophilic Hydrogenophilus thermoluteolus
Sotaro Fujii1, Misa Masanari, Masaru Yamanaka
1a Graduate School of Biosphere Science , Hiroshima University , Higashi-Hiroshima , Japan.
Abstract:
Apo-cytochomes c without heme are usually unstructured. Here we showed that apo-form of thermophilic Hydrogenophilus thermoluteolus cytochrome c' (PHCP) was a monomeric protein with high helix content. Apo-PHCP was thermally stable, possibly due to the hydrophobic residues and ion pairs. PHCP is the first example of a structured apo-cytochrome c', which will expand our view of hemoprotein structure formation.
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