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Functional mapping of the zebrafish early embryo proteome and transcriptome
Asfa Alli Shaik1, Sheena Wee, Rachel Hai Xia Li
1Institute of Molecular and Cell Biology, Agency for Science, Technology and Research , 61 Biopolis Drive, 138673, Singapore.
Journal of Proteome Research
|September 18, 2014
Summary
This study quantifies the most zebrafish embryo proteins to date, revealing how gene expression and protein levels coordinate during early vertebrate development. Findings highlight regulatory mechanisms controlling embryonic growth.
Area of Science:
- Developmental Biology
- Proteomics
- Transcriptomics
Background:
- Zebrafish are a key model for vertebrate development and disease due to genetic similarity with humans.
- Understanding molecular processes during ontogeny is crucial for developmental studies.
Purpose of the Study:
- To conduct a comparative analysis of the whole proteome and transcriptome in early-stage zebrafish embryos.
- To identify and quantify proteins and transcripts to understand regulatory mechanisms in development.
Main Methods:
- Comparative analysis of whole proteome and transcriptome using mass spectrometry and deep sequencing.
- Extensive deyolking and fractionation procedures for protein identification.
- Integrative functional mapping and network analysis of quantified genes.
Main Results:
- Quantified 8363 zebrafish embryo proteins, the largest number reported to date.
- Found a moderate correlation between expressed proteome and transcriptome across cellular processes.
- Identified signal transduction pathways and potential post-transcriptional regulation in low-abundance proteins.
Conclusions:
- The study provides deep coverage of the functional proteome and transcriptome in developing zebrafish.
- Findings reveal differential exploitation of transcriptional and post-transcriptional regulation in embryonic development.
- Unveiled molecular regulatory mechanisms essential for vertebrate embryonic development.
