Related Experiment Video

Updated: Apr 23, 2026

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
08:25

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Published on: June 5, 2020

6.7K

Genotoxic evaluation of terbinafine in human lymphocytes in vitro

Danielle Tolomeotti1, Marialba Avezum Alves de Castro-Prado, Juliane Rocha de Sant'Anna

  • 1Departamento de Biotecnologia, Genética e Biologia Celular and.

Drug and Chemical Toxicology
|September 19, 2014
PubMed

Insights

Terbinafine, an antifungal drug, was evaluated for genotoxic effects in human lymphocytes. The study found no evidence of genotoxicity or cytotoxicity at tested concentrations, suggesting safety for human cells.

Area of Science:

  • Toxicology
  • Genetics
  • Pharmacology

Background:

  • Terbinafine is an established antimycotic agent with emerging potential in cancer therapy.
  • Limited data exists on the genotoxic effects of terbinafine in mammalian cells.

Purpose of the Study:

  • To investigate the genotoxic potential of terbinafine in cultured human peripheral blood lymphocytes.
  • To assess terbinafine's effects on chromosomal aberrations and other genetic endpoints.

Main Methods:

  • Human peripheral blood lymphocytes were exposed to terbinafine at various concentrations, including peak plasma levels.
  • Genetic endpoints analyzed included chromosomal aberrations (CA), sister chromatid exchanges (SCE), micronuclei (MN), nucleoplasmic bridges (NP), and nuclear buds (NB).
  • Statistical analysis using the Kruskal-Wallis test was performed to determine significance.

Main Results:

  • No significant differences were observed in CA, SCE, MN, NP, or NB frequencies at any tested concentration (p > 0.05).
  • Mitotic, replication, and cytokinesis-block proliferation indices remained unaffected by terbinafine exposure.
  • The results indicated no genotoxic or cytotoxic effects of terbinafine under the experimental conditions.

Conclusions:

  • Terbinafine demonstrated a lack of genotoxic and cytotoxic activity in cultured human peripheral blood lymphocytes.
  • These findings suggest terbinafine is safe concerning genotoxicity in human lymphocytes at the tested concentrations.
Keywords:
Antifungal agentchromosomal aberrationsmicronuclei assaysister chromatid exchanges

More Related Videos

The Lambda Select cII Mutation Detection System07:08

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

7.5K
Cytotoxicity Assays with Zebrafish Cell Lines08:22

Cytotoxicity Assays with Zebrafish Cell Lines

Published on: January 6, 2023

8.1K

Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

4.7K
Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
2.0K
Toxicity Testing in Animals01:23

Toxicity Testing in Animals

Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
198