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Related Experiment Video

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Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Graphene oxide-based SPR biosensor chip for immunoassay applications.

Nan-Fu Chiu¹, Teng-Yi Huang¹, Hsin-Chih Lai²

¹Institute of Electro-Optical Science and Technology, National Taiwan Normal University, No. 88, Sec. 4, Ting-Chou Road, Taipei 11677, Taiwan.

Nanoscale Research Letters

|September 19, 2014

Summary

This study introduces a sensitive graphene oxide sheet (GOS)-based surface plasmon resonance (SPR) sensor. The GOS chip demonstrates significantly higher sensitivity and a lower detection limit for bovine serum albumin (BSA) compared to traditional sensors.

Keywords:

Bovine albumin serum (BSA)Graphene oxide sheet (GOS)Protein Surface plasmon resonance (SPR)

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

Biomedical Engineering
Materials Science
Analytical Chemistry

Background:

Surface Plasmon Resonance (SPR) is a label-free optical sensing technique.
Conventional SPR sensors often utilize gold (Au) films, which can have limitations in sensitivity.
Developing novel materials for enhanced SPR sensing is crucial for diagnostics.

Purpose of the Study:

To develop a highly sensitive immunoassay sensor using graphene oxide sheets (GOS) for SPR chips.
To chemically modify GOS surfaces to enhance interaction with target analytes like bovine serum albumin (BSA).
To compare the performance of the GOS-based SPR sensor with conventional Au-film-based SPR sensors.

Main Methods:

Chemically modifying graphene oxide sheet (GOS) surfaces to create a sensing film.
Utilizing GOS-based SPR chips for detecting bovine serum albumin (BSA) and anti-BSA.
Measuring detection limits, affinity constants (K A), and SPR angle shifts.

Main Results:

The GOS film-based SPR chip exhibited a BSA detection limit 100 times lower than conventional Au-film sensors.
The affinity constant (K A) for BSA on the GOS chip was 5.2 times higher than on the conventional chip.
The GOS-based SPR sensor showed enhanced SPR angle shifts for both low and high concentrations of anti-BSA.

Conclusions:

Graphene oxide sheets (GOS) are a promising material for developing highly sensitive SPR-based immunoassay sensors.
The chemically modified GOS film offers superior sensitivity and binding affinity for protein detection.
This GOS-based SPR sensor technology holds potential for advanced clinical diagnostic applications.