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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
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LncRBase: an enriched resource for lncRNA information.

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  • 1Bioinformatics Centre, Bose Institute, Kolkata, India.

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|September 19, 2014
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Summary

Researchers developed LncRBase, a new database classifying and characterizing long noncoding RNAs (lncRNAs) in humans and mice. This resource aids in understanding the complex origins and functions of these crucial regulatory molecules.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Long noncoding RNAs (lncRNAs) are key regulators in cellular processes, but their functional annotation remains challenging.
  • Ongoing classification efforts highlight the complexity and ambiguity surrounding lncRNA biogenesis and function.
  • Understanding lncRNA genomic origin and molecular features is crucial for deciphering their roles.

Purpose of the Study:

  • To develop LncRBase, a comprehensive database for classifying and characterizing human and mouse lncRNAs.
  • To provide a detailed resource on lncRNA subtypes, genomic context, and interactions.
  • To facilitate research into the origin and function of lncRNAs.

Main Methods:

  • Development of the LncRBase database.
  • Classification and characterization of lncRNA transcripts in human and mouse genomes.
  • Annotation of novel lncRNAs from existing genomic datasets (UCSC, H-InvDB 8.0).
  • Inclusion of lncRNA variants from protein-coding gene loci.
  • Integration of data on lncRNA interactions with small noncoding RNAs (piRNAs, miRNAs) and genomic elements.

Main Results:

  • LncRBase contains 83,201 mouse and 133,361 human lncRNA entries across fourteen subtypes.
  • Newly annotated 8,507 mouse and 14,813 human noncoding RNA transcripts as lncRNAs.
  • Database includes lncRNA transcript variants hosted by protein-coding genes.
  • Provides information on genomic context, interactions with piRNAs and miRNAs, and regulatory mechanisms.

Conclusions:

  • LncRBase offers a valuable resource for studying lncRNA complexity, origin, and function.
  • The database enhances understanding of lncRNA genomic context and molecular interactions.
  • LncRBase provides a user-friendly interface for accessing comprehensive lncRNA information.