Related Experiment Video
Updated: Apr 23, 2026
09:37
An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
3.1K
Long non-coding RNAs as a source of new peptides
Jorge Ruiz-Orera1, Xavier Messeguer2, Juan Antonio Subirana1
1Evolutionary Genomics Group, Research Programme on Biomedical Informatics, Hospital del Mar Research Institute, Universitat Pompeu Fabra, Barcelona, Spain.
Elife
|September 19, 2014
Summary
Long non-coding RNAs (lncRNAs) may serve as a source for new peptide synthesis. Ribosome association suggests lncRNAs can be translated into short peptides, aiding de novo protein evolution.
Area of Science:
- Genomics
- Molecular Biology
- Evolutionary Biology
Background:
- Deep transcriptome sequencing identified numerous long non-coding RNAs (lncRNAs) lacking long or conserved open reading frames (ORFs).
- The function of most lineage-specific lncRNAs remains largely unknown.
- lncRNAs are hypothesized to act as a reservoir for novel peptide synthesis.
Purpose of the Study:
- To investigate the potential role of lncRNAs in the synthesis of new peptides.
- To determine if lncRNAs are translated into functional peptides.
Main Methods:
- Deep transcriptome sequencing across six species.
- Analysis of ribosome association with lncRNAs.
- Assessment of coding potential and sequence constraints of lncRNAs.
Main Results:
- A significant proportion of expressed lncRNAs in six species were found to be associated with ribosomes.
- Ribosome protection patterns indicated the translation of short peptides from lncRNAs.
- lncRNAs exhibit coding potential and sequence constraints comparable to young protein-coding sequences.
Conclusions:
- lncRNAs are actively translated into short peptides.
- lncRNAs play a significant role in de novo protein evolution.
- lncRNAs represent a novel source of evolutionary innovation in protein synthesis.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
7.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
7.5K
02:39lncRNA - Long Non-coding RNAs
2.6K
2.6K
02:53Ribosomal RNA Synthesis
12.3K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
12.3K
01:20Types of RNA
13.9K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
13.9K
01:23Types of RNA
61.1K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
61.1K
02:24Ribosome Profiling
3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K