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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.3K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.3K
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

4.0K
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
4.0K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.0K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2.0K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

897
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
897
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

1.4K
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
1.4K
Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

6.7K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
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Updated: Apr 23, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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A Strategy for Sensitive, Large Scale Quantitative Metabolomics

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[Quantitative metabolomics based on NMR].

Chun-Ying Jiang, Ying-Hong Wang

    Yao Xue Xue Bao = Acta Pharmaceutica Sinica
    |September 20, 2014
    PubMed
    Summary
    This summary is machine-generated.

    Quantitative nuclear magnetic resonance (qNMR) is a powerful tool for identifying and quantifying chemicals in complex mixtures. This review explores its applications in quantitative metabolomics for bioanalysis, highlighting strengths, limitations, and practical considerations.

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    Area of Science:

    • Analytical Chemistry
    • Biochemistry
    • Spectroscopy

    Background:

    • Nuclear Magnetic Resonance (NMR) spectroscopy is a versatile technique for chemical analysis.
    • Advances in NMR technology have enabled quantitative NMR (qNMR) for precise chemical measurements.
    • qNMR is increasingly applied to biological samples for metabolomics studies.

    Purpose of the Study:

    • To review the strengths and limitations of NMR-based quantitative metabolomics.
    • To discuss practical considerations for acquiring qNMR data in bioanalysis.
    • To present recent applications of qNMR in metabolomics.

    Main Methods:

    • Quantitative Nuclear Magnetic Resonance (qNMR) spectroscopy.
    • Metabolomics and targeted metabolic profiling.
    • Bioanalysis of biological samples.

    Main Results:

    • qNMR offers a valuable analytical tool for identifying and quantifying chemicals in complex mixtures.
    • NMR-based quantitative metabolomics provides insights into biological sample composition.
    • Practical considerations are crucial for successful qNMR data acquisition in bioanalysis.

    Conclusions:

    • NMR-based quantitative metabolomics is a powerful approach for characterizing biological samples.
    • Understanding the strengths, limitations, and practical aspects of qNMR is essential for its effective application.
    • qNMR is a valuable technique with growing importance in metabolomics and bioanalysis.