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Related Experiment Videos

Transferable vancomycin and teicoplanin resistance in Enterococcus faecium.

R Leclercq1, E Derlot, M Weber

  • 1Service de Bactériologie-Virologie-Hygiène, Hôpital Henri Mondor, Université Paris XII, Créil, France.

Antimicrobial Agents and Chemotherapy
|January 1, 1989
PubMed
Summary

This study reports transferable glycopeptide resistance in Enterococcus faecium BM4165, a strain also resistant to MLS antibiotics. This transferable resistance raises concerns about the spread of antibiotic resistance in gram-positive bacteria.

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Area of Science:

  • Microbiology
  • Antibiotic Resistance Research
  • Molecular Biology

Background:

  • Enterococcus faecium strains BM4165 and BM4178 exhibit resistance to vancomycin and teicoplanin.
  • These strains were isolated from immunocompromised patients, with one patient having received vancomycin treatment.
  • Strain BM4165 also displays resistance to macrolide-lincosamide-streptogramin B (MLS) antibiotics.

Purpose of the Study:

  • To investigate the genetic basis of antibiotic resistance in Enterococcus faecium strains BM4165 and BM4178.
  • To determine the transferability of glycopeptide and MLS resistance genes between bacterial strains.
  • To assess the potential for dissemination of glycopeptide resistance among gram-positive bacteria.

Main Methods:

  • Isolation and characterization of Enterococcus faecium strains BM4165 and BM4178.

Related Experiment Videos

  • Plasmid analysis to identify genes responsible for glycopeptide and MLS resistance.
  • Conjugation experiments to assess the self-transferability of resistance plasmids to various bacterial species.

    • Main Results:

    • Genes conferring resistance to glycopeptides and MLS antibiotics in strain BM4165 are located on plasmids pIP819 and pIP821.
    • Plasmid pIP819, carrying both glycopeptide and MLS resistance genes, is self-transferable to E. faecium and other gram-positive species, including Listeria monocytogenes.
    • Inducible glycopeptide resistance was conferred to E. faecium, Streptococcus species, and Listeria monocytogenes, but not to Staphylococcus aureus.

    Conclusions:

    • This study presents the first report of transferable glycopeptide resistance in Enterococcus faecium.
    • The findings indicate a significant risk for the dissemination of glycopeptide resistance among gram-positive cocci and bacilli.
    • The biochemical mechanism of glycopeptide resistance remains to be elucidated.