Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

7.1K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
7.1K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

8.2K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
8.2K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.1K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antigen-presenting cancer-associated fibroblasts in murine pancreatic tumors differentially regulate T-cell phenotype and function.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

A STAT1/ETC/GBP1 axis represents a potential therapeutic target for noncommunicable granulomatous skin disease.

Science advances·2026
Same author

Cardiolipin preserves T<sub>reg</sub> metabolic fitness and immune homeostasis in the gut.

Nature metabolism·2026
Same author

IL-33 promotes transcriptional and metabolic adaptations of tissue-resident Th2 cells.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Monocytes are biological sensors of aging and frailty in humans.

bioRxiv : the preprint server for biology·2026
Same author

FLI-ing the script on NK cell persistence: Proteostasis as a limiting factor.

Immunity·2026

Related Experiment Video

Updated: Apr 23, 2026

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
07:42

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Published on: June 12, 2013

18.7K

For macrophages, Ndufs is enough.

Stanley Ching-Cheng Huang1, Edward J Pearce1

  • 1Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO63110-1093, USA.

Immunity
|September 20, 2014
PubMed
Summary
This summary is machine-generated.

Proinflammatory macrophage activation involves a metabolic shift to glycolysis. Mitochondrial electron transport chain complex I negatively regulates this process via intrinsic and extrinsic pathways, impacting cellular metabolism.

More Related Videos

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
06:46

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Published on: June 30, 2016

30.1K
Depletion and Reconstitution of Macrophages in Mice
08:50

Depletion and Reconstitution of Macrophages in Mice

Published on: August 1, 2012

39.2K

Related Experiment Videos

Last Updated: Apr 23, 2026

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
07:42

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Published on: June 12, 2013

18.7K
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
06:46

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Published on: June 30, 2016

30.1K
Depletion and Reconstitution of Macrophages in Mice
08:50

Depletion and Reconstitution of Macrophages in Mice

Published on: August 1, 2012

39.2K

Area of Science:

  • Cellular metabolism
  • Immunometabolism
  • Mitochondrial function

Background:

  • Macrophage activation during inflammation is linked to metabolic reprogramming.
  • Glycolysis is a key metabolic pathway utilized by activated macrophages.

Purpose of the Study:

  • To investigate the role of mitochondrial electron transport chain complex I in regulating the metabolic switch during macrophage activation.
  • To elucidate the pathways through which complex I exerts its regulatory effects.

Main Methods:

  • Analysis of macrophage activation markers.
  • Metabolic flux analysis to assess glycolysis rates.
  • Investigating mitochondrial electron transport chain complex I activity.
  • Exploring cell-intrinsic and cell-extrinsic regulatory mechanisms.

Main Results:

  • Proinflammatory macrophage activation is associated with increased glycolysis.
  • Mitochondrial electron transport chain complex I activity negatively correlates with glycolytic rates.
  • Complex I regulates this metabolic switch through both cell-intrinsic and extrinsic mechanisms.

Conclusions:

  • Mitochondrial electron transport chain complex I acts as a negative regulator of the glycolytic switch in activated macrophages.
  • Understanding this regulation provides insights into immunometabolism and potential therapeutic targets.