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Updated: Apr 23, 2026

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
Deviations of inorganic and organic carbon content in hypomineralised enamel
F Taube1, M Marczewski2, J G Norén3
1Department of Occupational and Environmental Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden.
Objectives:
The purpose of this study was to discriminate hypomineralised enamel of permanent first molars from normal enamel by means of spectroscopic methods.
Methods:
The present study was conducted using Multi spot Raman Fourier Transform Spectroscopy, Diffuse Reflectance Infrared Fourier Transform Spectroscopy (FTIR) and X-ray diffraction (XRD).
Results:
Raman spectroscopy indicated significantly more B-type carbonate and hydrocarbons in hypomineralised enamel diagnosed as MIH (Molar Incisor Hypomineralisation). From XRD analysis, no changes in crystallinity of the enamel apatite could be found.
Conclusions:
Using multi spot Raman-spectroscopy, a significant molecular discrimination between normal and hypomineralised enamel could be made.
Clinical Significance:
Detailed surface studies are needed in order to achieve better restorative materials, specifically designed for restoration of hypomineralised enamel, and are also needed in order to understand and predict the clinical consequences of hypomineralised enamel with the condition MIH.
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