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Detection of cyclic di-AMP using a competitive ELISA with a unique pneumococcal cyclic di-AMP binding protein
Adam J Underwood1, Yang Zhang1, Dennis W Metzger1
1Center for Immunology and Microbial Disease, MC-151, Albany Medical College, Albany, NY 12208-3479, USA.
Journal of Microbiological Methods
|September 21, 2014
Abstract:
Cyclic di-AMP (c-di-AMP) is a recently recognized bacterial signaling molecule. In this study, a competitive enzyme-linked immunosorbent assay (ELISA) for the quantification of c-di-AMP was developed using a novel pneumococcal c-di-AMP binding protein (CabP). With this method, c-di-AMP concentrations in biological samples can be quickly and accurately quantified.

