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A ratiometric fluorescent probe based on a coumarin-hemicyanine scaffold for sensitive and selective detection of
Xin Zhou1, Younghee Kwon2, Gyoungmi Kim3
1Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea; Department of Chemistry, Faculty of Science, Yanbian University, People's Republic of China.
Abstract:
In the study described herein, the red emitting probe CHCN, which possesses a linked coumarin-hemicyanine scaffold, was developed for detection of peroxynitrite (ONOO(-)) under physiological conditions. The studies show that CHCN displays a dual ratiometric and colorimetric response to ONOO(-) that is caused by an oxidation process. A possible mechanism of this oxidation process was proposed and confirmed by ESI-MS spectra for the first time. CHCN shown highly selective and sensitive towards ONOO(-) with a low limit of detection LOD (49.7 nM). Moreover, CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO(-) in living cells during phagocytic immune response. We anticipate that, owing to their ideal properties, probes of this type will find great use in explorations of the role played by ONOO(-) in biology.
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