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Nucleotide sequence and structural organization of the human FMS proto-oncogene

A Hampe1, B M Shamoon, M Gobet

  • 1Laboratoire d'Hematologie Experimentale, Centre Hayem, Hospital Saint Louis, Paris, France.

Oncogene Research
|January 1, 1989
PubMed

Insights

The FMS gene encodes the CSF-1 receptor, a key protein in cell signaling. Sequencing revealed its complex structure, including a distant upstream exon, crucial for understanding its function.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • The human proto-oncogene c-fms (FMS) encodes a tyrosine kinase receptor for macrophage colony-stimulating factor (CSF-1/M-CSF).
  • Understanding the FMS gene structure is vital for its role in cell growth and differentiation.

Purpose of the Study:

  • To analyze the nucleotide sequence of the human c-fms gene locus.
  • To characterize the genomic organization of the CSF-1 receptor.

Main Methods:

  • Nucleotide sequencing of overlapping bacteriophage clones spanning 35 kb of the FMS locus.
  • Comparison of genomic DNA sequence with the known cDNA sequence of the human c-fms gene.

Main Results:

  • The complete coding sequence of the CSF-1 receptor was identified.
  • A 5' noncoding exon was located approximately 26 kb upstream of the coding sequence.
  • The FMS coding sequence comprises 21 small exons separated by introns of variable sizes.

Conclusions:

  • The FMS gene exhibits a complex genomic structure with a distantly located upstream exon.
  • This detailed structural information provides a foundation for further studies on FMS gene regulation and function.

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