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Updated: Apr 23, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Sequence-defined oligoaminoamides for the delivery of siRNAs
Dian-Jang Lee1, Ernst Wagner, Taavi Lehto
1Department of Pharmacy and Center for Nanoscience (CeNS), Pharmaceutical Biotechnology, Center for System-Based Drug Research, Ludwig Maximilians University of Munich, Building D, Butenandtstraße 5-13, Munich, 81377, Germany.
Abstract:
Since it was found that synthetic small interfering RNA (siRNA) can invoke RNA interference (RNAi) responses in mammalian cells, it has gained enormous attention as a tool for gene silencing in basic science and as a novel therapeutic modality. To develop carriers for cytosolic and systemic siRNA delivery, our laboratory has recently developed a sequence-defined polymer platform compatible with solid-phase-supported synthesis. These polymers have displayed efficient siRNA-mediated gene silencing in vitro and in vivo. In this chapter, we provide a brief background on the special features of these polymers and detailed protocols to evaluate polyplex formation, gene silencing efficiency, and cytotoxicity of siRNA-containing polyplexes.
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