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Proteome changes in mesenteric lymph induced by sepsis.

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Sepsis development significantly alters mesenteric lymph proteins, with five key proteins (apolipoprotein E, annexin A1, neutrophil gelatinase-associated lipocalin, S100a8, and S100a9) showing elevated expression and potential as sepsis biomarkers.

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Area of Science:

  • Biochemistry
  • Pathology
  • Proteomics

Background:

  • Sepsis is a life-threatening condition characterized by a dysregulated host response to infection.
  • Mesenteric lymph plays a crucial role in the immune response and fluid balance.
  • Understanding proteomic changes in mesenteric lymph during sepsis is vital for identifying diagnostic and therapeutic targets.

Purpose of the Study:

  • To investigate proteomic alterations in mesenteric lymph during sepsis progression.
  • To identify specific proteins in mesenteric lymph that change during sepsis.
  • To evaluate the potential of these proteins as biomarkers for sepsis diagnosis and treatment.

Main Methods:

  • A sepsis animal model was established using cecal ligation and puncture (CLP) in Sprague-Dawley rats.
  • Mesenteric lymph was collected from CLP and sham-operated groups at 6 and 24 hours post-procedure.
  • Capillary high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed for proteomic analysis, followed by bioinformatic analysis.

Main Results:

  • A total of 158 distinct proteins were identified in the mesenteric lymph of septic rats compared to controls.
  • Five proteins—apolipoprotein E (ApoE), annexin A1 (Anxa1), neutrophil gelatinase-associated lipocalin (NGAL), S100a8, and S100a9—were significantly elevated during sepsis progression.
  • These five proteins are involved in lipid metabolism and inflammatory pathways.

Conclusions:

  • Proteomic analysis of mesenteric lymph reveals significant changes during sepsis development.
  • The identified proteins, particularly ApoE, Anxa1, NGAL, S100a8, and S100a9, represent potential novel biomarkers for sepsis.
  • Targeting these proteins may offer new avenues for sepsis diagnosis and therapeutic intervention.