Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SED88: a Pascal program for the analysis of sedimentation equilibrium data.

P M Reid1, A E Wilkinson, K C Leung

  • 1Department of Biochemistry and Molecular Biology, University of Manchester, UK.

Computer Applications in the Biosciences : CABIOS
|April 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Music-paced physical activity intervention for patients with coronary heart disease: abridged secondary publication.

Hong Kong medical journal = Xianggang yi xue za zhi·2024
Same author

A territory-wide real-world efficacy and toxicity analysis of abiraterone acetate versus docetaxel in 574 Asian patients with metastatic hormone-sensitive prostate cancer.

Clinical genitourinary cancer·2023
Same author

Measuring disorganized speech in schizophrenia: automated analysis explains variance in cognitive deficits beyond clinician-rated scales.

Psychological medicine·2018
Same author

Simultaneous pancreas and kidney transplantation as the standard surgical treatment for diabetes mellitus patients with end-stage renal disease.

Hong Kong medical journal = Xianggang yi xue za zhi·2016
Same author

Attitude toward the out-patient cardiac rehabilitation program and facilitators for maintenance of exercise behavior.

Psychology, health & medicine·2015
Same author

Docetaxel chemotherapy for Chinese patients with castrate-resistant prostate cancer.

Hong Kong medical journal = Xianggang yi xue za zhi·2013

This study presents a Turbo Pascal program for analyzing sedimentation equilibrium centrifugation data to determine the molecular weight of biological macromolecules. The software, usable with an IBM PC and graphics tablet, was applied to International Humic Substances Society reference samples.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Computational Biology

Background:

  • Analytical ultracentrifugation is a key technique for determining molecular weights and sedimentation coefficients of biological macromolecules.
  • Accurate molecular weight determination is crucial for understanding macromolecular behavior in solution.

Purpose of the Study:

  • To describe a Turbo Pascal program for analyzing sedimentation equilibrium centrifugation data.
  • To provide a user-friendly method for calculating apparent weight-average molecular weight using readily available computational equipment.

Main Methods:

  • Sedimentation equilibrium centrifugation data acquisition via absorbance optical systems.
  • Data input using a graphics tablet or ASCII file.
  • Software-based analysis to compute apparent weight-average molecular weight and generate plots.

Related Experiment Videos

Main Results:

  • Development and validation of a Turbo Pascal program for sedimentation equilibrium data analysis.
  • Successful application of the software to determine the molecular weight range of IHSS reference samples from the Suwannee River.

Conclusions:

  • The described method and software offer an accessible approach for molecular weight determination using standard laboratory equipment.
  • This computational tool facilitates the investigation of macromolecular properties, as demonstrated with humic substances.