Related Experiment Video
Updated: Apr 23, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
Yun-Gang Jiang1, Chu-Yan Wang2, Chao Jin1
1Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China.
Abstract:
DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf.
Related Concept Videos
Bioreactor Controls-III
Production of Organic Acids
Microbial Fermentation
Microbes in the Production of Fermented Foods
Production of Antibiotics
Bioremediation

