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[Molecular identification of Manis pentadactyla using DNA barcoding].

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    The cytochrome c oxidase subunit I (COI) gene effectively identifies the Chinese pangolin (Manis pentadactyla) and its substitutes. This molecular method provides a reliable tool for market supervision and species authentication.

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    Area of Science:

    • Molecular Biology
    • Zoology
    • Forensic Science

    Background:

    • Accurate identification of Manis pentadactyla is crucial for conservation and market regulation.
    • Existing identification methods may lack molecular precision.
    • The need for a robust DNA-based identification system is evident.

    Purpose of the Study:

    • To establish the cytochrome c oxidase subunit I (COI) gene as a DNA barcode for identifying Manis pentadactyla.
    • To differentiate M. pentadactyla from its common adulterants using molecular techniques.
    • To provide a scientific foundation for the molecular identification of pangolin species.

    Main Methods:

    • Genomic DNA extraction from experimental samples.
    • Amplification of COI gene sequences using polymerase chain reaction (PCR).
    • Bi-directional sequencing, sequence assembly (CodonCode Aligner), and phylogenetic analysis (Neighbor-Joining tree using MEGA 6.0).

    Main Results:

    • Successful amplification and sequencing of COI genes from M. pentadactyla and adulterant samples.
    • Neighbor-Joining tree analysis clearly distinguished M. pentadactyla from its adulterants.
    • Demonstrated the efficacy of COI sequences in species identification.

    Conclusions:

    • The COI gene serves as an efficient and reliable DNA barcode for the molecular identification of Manis pentadactyla.
    • This method offers a novel technique for market supervision and combating illegal wildlife trade.
    • Supports the development of advanced molecular tools for biodiversity monitoring and enforcement.