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Micro/nano-computed tomography technology for quantitative dynamic, multi-scale imaging of morphogenesis.

Chelsea L Gregg1, Andrew K Recknagel, Jonathan T Butcher

  • 1Department of Biomedical Engineering, Cornell University, 304 Weill Hall, Ithaca, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2014
PubMed
Summary

Micro-computed tomography (micro-CT) and nano-computed tomography (nano-CT) offer high-resolution 3D imaging for studying embryonic development. These techniques, enhanced by contrast agents, enable quantitative analysis of tissue morphogenesis and gene expression in live embryos.

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Area of Science:

  • Developmental Biology
  • Imaging Science
  • Biomedical Engineering

Background:

  • Tissue morphogenesis and embryonic development involve complex, simultaneous events challenging to quantify.
  • Micro-computed tomography (micro-CT) and nano-computed tomography (nano-CT) provide high-resolution 3D imaging for biological samples.
  • Existing imaging strategies require advancement for precise quantification of developmental changes.

Purpose of the Study:

  • To present established protocols for high-quality micro/nanoCT imaging of embryonic tissues.
  • To detail image processing techniques for quantifying anatomical and physiological data.
  • To highlight the utility of contrast-enhanced micro/nanoCT for studying developmental processes.

Main Methods:

  • Utilized micro/nanoCT for high spatial resolution 3D imaging of biological structures.
  • Employed exogenous soft tissue contrast media for enhanced visualization of lumens and tissues.
  • Applied antigen-specific tissue contrast for quantitative visualization of protein and mRNA expression.

Main Results:

  • Demonstrated successful application of micro/nanoCT for characterizing complex 3D geometries in developing tissues.
  • Showcased contrast-enhanced imaging for detailed visualization of vascular networks and tissue structures.
  • Indicated that micro-CT X-ray doses are non-embryotoxic, permitting longitudinal studies in live embryos.

Conclusions:

  • Micro/nanoCT imaging, with advanced contrast protocols, is a powerful tool for quantitative analysis of tissue morphogenesis.
  • These imaging strategies enable detailed assessment of anatomical and physiological changes during embryonic development.
  • The non-embryotoxic nature of micro-CT supports its use in longitudinal studies of live developing organisms.