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Related Experiment Videos

Host response to autologous endothelial seeding.

S Hussain1, J L Glover, N Augelli

  • 1Department of Surgery, William Beaumont Hospital, Royal Oak, MI 48072.

Journal of Vascular Surgery
|May 1, 1989
PubMed
Summary

Seeding expanded polytetrafluoroethylene grafts with endothelial cells did not significantly improve graft patency or endothelial coverage compared to unseeded grafts in dogs. This suggests host cell proliferation may contribute to graft lining. Keywords: endothelial cells, expanded polytetrafluoroethylene grafts, graft patency.

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Area of Science:

  • Biomaterials Science
  • Vascular Surgery
  • Cell Biology

Background:

  • Endothelial cell seeding of Dacron and expanded polytetrafluoroethylene (ePTFE) grafts aims to create functional endothelial linings.
  • The origin of these endothelial cells (seeded vs. host-derived) and the effect of seeding on host cell proliferation remain unclear.

Purpose of the Study:

  • To investigate whether autologous endothelial cell seeding influences the endothelialization and patency of ePTFE grafts.
  • To serve as a preliminary step in determining the source of endothelial cells covering vascular grafts.

Main Methods:

  • Bilateral end-to-side aortoiliac ePTFE grafts (6 mm internal diameter) were implanted in 10 dogs.
  • One graft per animal was seeded with autologous venous endothelial cells; the contralateral graft served as an unseeded control.

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  • Graft patency and endothelial coverage were assessed after 4 weeks using scanning electron microscopy.

    • Main Results:

    • No significant difference in clot-free surface area was observed between seeded (29% ± 18%) and unseeded (31% ± 11%) grafts.
    • Average endothelial monolayer coverage in patent grafts was similar: 39% ± 20% for seeded and 36% ± 26% for unseeded.
    • Graft patency rates showed no statistically significant advantage for seeded grafts over unseeded controls.

    Conclusions:

    • Autologous endothelial cell seeding of ePTFE grafts did not enhance graft patency or endothelial coverage in this canine model.
    • The findings suggest that host endothelial cells may play a significant role in covering both seeded and unseeded ePTFE grafts.
    • Further research is needed to elucidate the mechanisms of graft endothelialization and the contribution of seeded cells versus host cells.