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Updated: Apr 23, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Recent advances in stable isotope labeling based techniques for proteome relative quantification.

Yuan Zhou¹, Yichu Shan¹, Lihua Zhang¹

¹National Chromatographic R. & A. Center, Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Journal of Chromatography. A

|September 24, 2014

Summary

Accurate protein quantification using stable isotope labeling is crucial for discovering biological functions and disease biomarkers. This review covers advances in metabolic, chemical, and enzyme-catalyzed labeling techniques for proteome-wide relative quantification.

Keywords:

Chemical labeling Enzyme-catalyzed labeling Metabolic labeling Proteome Relative quantification Stable isotope labeling

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Last Updated: Apr 23, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

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Published on: August 17, 2015

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Proteomics
Biochemistry
Molecular Biology

Background:

Large-scale relative quantification of proteins across different biological states is vital for identifying functional proteins.
Accurate proteome-level protein quantification is a key challenge in contemporary protein science.
Identifying disease biomarkers and drug targets relies heavily on understanding protein expression levels.

Purpose of the Study:

To review recent advancements in stable isotope labeling techniques for proteome relative quantification.
To provide a comprehensive overview of metabolic, chemical, and enzyme-catalyzed labeling strategies.
To prospect future research directions in the field of quantitative proteomics.

Main Methods:

Review of literature on stable isotope labeling techniques.
Categorization of labeling methods into metabolic, chemical, and enzyme-catalyzed approaches.
Analysis of the strengths and limitations of each labeling strategy.

Main Results:

Detailed overview of various stable isotope labeling methods for quantitative proteomics.
Comparison of metabolic, chemical, and enzyme-catalyzed labeling in terms of efficiency, scope, and applicability.
Identification of key trends and challenges in current proteome quantification technologies.

Conclusions:

Stable isotope labeling techniques are essential tools for relative protein quantification in biological research.
Continued innovation in labeling methodologies will enhance the discovery of biomarkers and drug targets.
Future research should focus on improving labeling efficiency, accuracy, and applicability across diverse biological systems.