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Updated: Apr 23, 2026
Modeling Hypoxia/Reoxygenation Injury in Proximal Tubular Epithelial Cells
Published on: November 21, 2025
The protective effect of ozone oxidative preconditioning against hypoxia/reoxygenation injury in rat kidney cells
Lei Wang1, Hui Chen, Xiu-Heng Liu
1Department of Urology, Renmin Hospital of Wuhan University , Wuhan, Hubei Province , P.R. China.
Abstract:
Abstract Ozone (O3) has been viewed as a novel treatment for different diseases in these years and oxidative stress and apoptosis play a key role in the pathogenesis of kidney diseases including renal ischemia and reperfusion (I/R). In the present study, we investigated the role of ozone oxidative preconditioning (OzoneOP) in attenuating oxidative stress and apoptosis in a hypoxia/reoxygenation (H/R) injury model using rat kidney cells. We induced H/R injury in kidney cells treated with or without OzoneOP. Oxidative stress parameters such as superoxide dismutase (SOD), malondialdehyde (MDA) and lactate dehydrogenase (LDH) were determined, as well as some apoptotic proteins. We observed that oxidative stress and apoptosis were increased in H/R group compared to OzoneOP group; however, these changes were significantly decreased by the treatment with OzoneOP. We concluded that OzoneOP can protect the kidney cells against H/R injury and its mechanism may be through the reduction of oxidative stress and apoptosis.
Insights
Ozone oxidative preconditioning (OzoneOP) protects kidney cells from hypoxia/reoxygenation (H/R) injury. This novel treatment reduces oxidative stress and apoptosis, offering a potential therapeutic strategy for kidney diseases.
Area of Science:
- Nephrology
- Cell Biology
- Oxidative Stress Research
Background:
- Kidney diseases, including renal ischemia-reperfusion (I/R) injury, are often linked to oxidative stress and apoptosis.
- Ozone (O3) is emerging as a potential therapeutic agent for various conditions.
Purpose of the Study:
- To investigate the protective effects of ozone oxidative preconditioning (OzoneOP) against hypoxia/reoxygenation (H/R) induced kidney cell injury.
- To elucidate the role of OzoneOP in modulating oxidative stress and apoptosis in a rat kidney cell model.
Main Methods:
- Induction of H/R injury in rat kidney cells.
- Treatment of cells with or without OzoneOP.
- Measurement of oxidative stress markers (superoxide dismutase, malondialdehyde, lactate dehydrogenase) and apoptotic proteins.
Main Results:
- H/R injury significantly increased oxidative stress and apoptosis in kidney cells.
- OzoneOP treatment markedly decreased the levels of oxidative stress markers and apoptotic proteins.
- OzoneOP demonstrated a significant protective effect against H/R-induced kidney cell damage.
Conclusions:
- Ozone oxidative preconditioning (OzoneOP) effectively protects kidney cells against hypoxia/reoxygenation (H/R) injury.
- The protective mechanism of OzoneOP involves the reduction of oxidative stress and apoptosis.
- OzoneOP presents a promising therapeutic approach for mitigating kidney cell damage.
