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Prevalence of Bacterial Contamination when using a Diversion Pouch during Blood Collection: A Single Center Study in
Norlaili Jumaah1, Sanmukh Ratilal Joshi1, Doblin Sandai1
1Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam 13200 Kepala Batas, Penang, Malaysia.
Background:
The implementation of diversion pouches is to minimise the risk of bacterial contamination as the initial blood flow is prevented from entering primary bag collections as it is diverted into a pouch. This study was carried out to determine the prevalence of bacterial contamination in the diversion pouches used during blood collections in the Transfusion Department of Hospital Seberang Jaya, Penang, Malaysia.
Methods:
BD Bactec™ Fx instrument detection system was performed on 702 samples of 20 mL of diverting blood in diversion pouch. The inocullum volume was 10 mL for both aerobic and anaerobic bottles cultures and incubated for 5 days in the BD Bactec™ Fx instrument. Positive sample was flagged by BD Bactec™ Fx instrument and subculture to identify the species of organism.
Results:
The results showed that of 702 samples, 12 (1.7%) were contaminated. The bacterial species identified were coagulase negative Staphylococcus, Staphylococcus aureus and Gram positive Bacilli.
Conclusion:
The results strongly suggest that the usage of diversion pouch is of significant importance in reducing bacterial contamination during blood collection.
Insights
Diversion pouches significantly reduce bacterial contamination during blood collection. This study found only 1.7% contamination in diversion pouches, highlighting their importance in transfusion safety.
Area of Science:
- Transfusion Medicine
- Microbiology
- Infection Control
Background:
- Bacterial contamination is a risk in blood collection.
- Diversion pouches aim to minimize this risk by diverting initial blood flow.
- This study assessed contamination rates in diversion pouches at Hospital Seberang Jaya.
Purpose of the Study:
- To determine the prevalence of bacterial contamination in diversion pouches.
- To evaluate the effectiveness of diversion pouches in preventing contamination during blood collection.
Main Methods:
- 702 blood samples collected in diversion pouches were analyzed.
- BD Bactec™ Fx instrument detection system was used for bacterial detection.
- Positive samples were subcultured to identify bacterial species.
Main Results:
- A low contamination rate of 1.7% (12 out of 702 samples) was observed.
- Identified bacterial contaminants included coagulase-negative Staphylococcus, Staphylococcus aureus, and Gram-positive Bacilli.
Conclusions:
- The use of diversion pouches is crucial for reducing bacterial contamination in blood collection.
- Diversion pouches play a significant role in enhancing transfusion safety.
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