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Updated: Apr 23, 2026
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
Published on: December 3, 2011
Identification of several clades of novel single-stranded circular DNA viruses with conserved stem-loop structures in
Andrew K Cheung1, Terry Fei Fan Ng, Kelly M Lager
1Virus and Prion Diseases Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA, 50010, USA, Andrew.Cheung@ars.usda.gov.
Abstract:
Metagenomic analysis of fecal samples collected from diarrheal swine detected sequences encoding a replication initiator protein (Rep). The genomes of ten novel single-stranded DNA viruses were determined, and they exhibited a similar genome organization. The two putative open reading frames (ORFs) encoding Rep and the capsid protein are bidirectionally transcribed and separated by two intergenic regions. Stem-loop structure(s) typical of genomes that undergo the rolling-circle DNA replication mechanism were observed. Phylogenetically, these ten genomes are in a monophyletic clade with the previously described porcine stool-associated virus (PoSCV) but are divergent enough to be further classified into to six distinct virus clades.
