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Updated: Jan 24, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Published on: November 1, 2019
Halo-tag mediated self-labeling of fluorescent proteins to molecular beacons for nucleic acid detection
Daniel Blackstock1, Wilfred Chen
1Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware 19716, USA. wilfred@udel.edu.
Abstract:
We report here the generation of a fluorescent protein (FP)-based dual molecular beacon (MB) system for nucleic acid detection. Halo-tag mediated conjugation was used for the site-specific decoration of MBs with two different FP fusions, thereby enabling easy detection of target sequences by fluorescence resonance energy transfer or FRET. Enhanced intracellular delivery was demonstrated by simply tethering a well-known TAT peptide sequence to the N-terminus of the fusion proteins.
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