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Chicken 15-kDa selenoprotein plays important antioxidative function in splenocytes.

Huijie Sun1, Tingquan Deng, Jiaxing Fu

  • 1College of Computer Science and Technology, Harbin Engineering University, Harbin, 150001, People's Republic of China.

Biological Trace Element Research
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Chicken 15-kDa selenoprotein (Sep15) shares high similarity with mammalian homologs. Selenium deficiency reduces Sep15 levels, and Sep15 deficiency increases oxidative stress, confirming its antioxidant role in chickens.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Animal Science

Background:

  • The 15-kDa selenoprotein (Sep15) is a thioredoxin-like protein crucial in mammals.
  • Dietary selenium (Se) regulates Sep15 expression, but its role in chickens remains unclear.

Purpose of the Study:

  • To investigate the structure, function, and selenium regulation of chicken Sep15.
  • To analyze the impact of Sep15 deficiency on oxidative stress in chicken splenocytes.

Main Methods:

  • Replication of chicken selenium deficiency and Sep15 deficiency models in splenocytes.
  • Analysis of Sep15 homology, structure, and expression levels.
  • Examination of oxidative stress markers in Sep15-deficient cells.

Main Results:

  • Chicken Sep15 exhibits high sequence similarity to homologs in 14 other animal species.
  • Predicted structure reveals a conserved thioredoxin-like fold (CxU), suggesting antioxidant function.
  • Selenium deficiency decreased chicken Sep15 levels in immune organs.
  • Sep15 deficiency in splenocytes led to increased oxidative stress and heightened sensitivity to hydrogen peroxide (H2O2).

Conclusions:

  • Chicken Sep15 shares conserved characteristics with mammalian selenoproteins.
  • Sep15 plays a significant role in antioxidant defense and redox regulation in chickens, similar to its mammalian counterparts.