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Related Experiment Video

Updated: Apr 23, 2026

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Published on: March 22, 2024

Transcriptomic analysis of mouse limb tendon cells during development.

Emmanuelle Havis¹, Marie-Ange Bonnin¹, Isabel Olivera-Martinez²

¹CNRS UMR 7622, IBPS-Developmental Biology Laboratory, Paris F-75005, France Sorbonne Universités, UPMC Univ Paris 06, IBPS-Developmental Biology Laboratory, Paris F-75005, France Inserm U1156, Paris F-75005, France.

Development (Cambridge, England)

|September 25, 2014

Summary

This summary is machine-generated.

Transforming growth factor-beta (TGF-β) signaling is crucial for tendon development, driving mesodermal stem cells toward the tendon lineage. Inhibition of MAPK signaling also activates key tendon development genes.

Keywords:

ERK Limb Mouse SMAD2/3 Scleraxis TGF-β Tendon Transcriptome

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Published on: August 1, 2020

Area of Science:

Molecular Biology
Developmental Biology
Stem Cell Biology

Background:

Tendon development is a complex process regulated by specific molecular signals.
The complete set of signals governing tendon formation remains incompletely understood.

Purpose of the Study:

To identify key molecular signals regulating mouse limb tendon cell development.
To elucidate the roles of TGF-β and MAPK signaling pathways in tenogenesis.

Main Methods:

Transcriptome analysis of mouse limb tendon cells at various developmental stages.
Bioinformatics analysis to identify significantly altered signaling pathways.
Gain- and loss-of-function experiments using mouse limb explants and mesenchymal stem cells.

Main Results:

Transcriptome analysis identified TGF-β and MAPK as the most significantly modified signaling pathways.
TGF-β signaling, mediated by SMAD2/3, is both sufficient and required for driving stem cells towards the tendon lineage.
Inhibition of the ERK MAPK pathway was sufficient to activate Scleraxis (Scx) in progenitor cells.

Conclusions:

TGF-β/SMAD2/3 signaling plays a critical role in directing mesodermal stem cells to adopt a tendon fate.
MAPK signaling, specifically ERK, negatively regulates tendon progenitor activation.
These findings provide crucial insights into the molecular mechanisms of tendon development.