Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Truncated forms of human complement factor H.

M Fontaine1, M J Demares, V Koistinen

  • 1I.N.S.E.R.M. Unité 78, Bois-Guillaume, France.

The Biochemical Journal
|March 15, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of a Ym1 deficient mouse utilising CRISPR-Cas9 in CB6 embryos.

Transgenic research·2025
Same author

In Vitro Percutaneous Absorption of Permeation-Enhancing Estrogen Formulations.

Pharmaceuticals (Basel, Switzerland)·2025
Same author

Physicochemical and Microbiological Stability of Ursodiol Oral Compounded Suspensions.

The journal of pediatric pharmacology and therapeutics : JPPT : the official journal of PPAG·2024
Same author

Cutaneous targets for topical pain medications in patients with neuropathic pain: individual differential expression of biomarkers supports the need for personalized medicine.

Pain reports·2024
Same author

Safety and efficacy of compounded bioidentical hormone therapy (cBHT) in perimenopausal and postmenopausal women: a systematic review and meta-analysis of randomized controlled trials.

Menopause (New York, N.Y.)·2022
Same author

Quality of life in colostomy patients practicing colonic irrigation: An observational study.

Journal of visceral surgery·2020
Same journal

Mechanistic insights into acetylated histone recognition by the CECR2 bromodomain.

The Biochemical journal·2026
Same journal

Nanobodies against Plasmodium adhesins that block receptor engagement and malaria parasite invasion.

The Biochemical journal·2026
Same journal

Persistence without turnover: the RhoG G12E mutant highlights the role of nucleotide cycling in RhoG signaling.

The Biochemical journal·2026
Same journal

Alternative Splicing of Rice Chloroplastic CuZn Superoxide Dismutase, OsCSD2: Impact on expression and protein characteristics.

The Biochemical journal·2026
Same journal

Difference and similarity between the ubiquitous secretory pathway Ca2+-ATPases, SERCA2b, and SPCA1a.

The Biochemical journal·2026
Same journal

A molecular perspective on dimethylarginine dimethylaminohydrolases structure and function.

The Biochemical journal·2026
See all related articles

Researchers identified two novel low-molecular-weight Factor H protein variants in human samples. These variants, distinct from known Factor H fragments, may originate from short Factor H mRNA species.

Area of Science:

  • Molecular Biology
  • Immunology
  • Proteomics

Background:

  • Factor H is a key regulator of the alternative complement pathway.
  • Previous research identified a 155,000-Mr Factor H in human plasma and serum.
  • The existence and origin of smaller Factor H protein species remained unclear.

Purpose of the Study:

  • To investigate the presence and characteristics of low-molecular-weight protein species associated with Factor H.
  • To determine the relationship of these species to known Factor H and its fragments.
  • To explore the potential origin of these novel Factor H variants.

Main Methods:

  • Western-blot analyses using polyclonal and monoclonal anti-(Factor H) antibodies.
  • Analysis under both non-reducing and reducing conditions.

Related Experiment Videos

  • Detection in human plasma, serum, urine, and synovial fluids.

    • Main Results:

    • Two distinct low-Mr protein species (41,000 and 37,000 under non-reducing conditions) were consistently detected alongside the 155,000-Mr Factor H.
    • These species were found in various human biological fluids.
    • Monoclonal antibodies indicated the 41,000-Mr species contains the N-terminus, while the 37,000-Mr species contains the C-terminus of Factor H, differentiating them from a known N-terminal tryptic fragment.

    Conclusions:

    • The identified 41,000-Mr and 37,000-Mr protein species represent novel forms of Factor H.
    • These variants are likely translational products derived from recently described short Factor H mRNA species (1.8 kb and 1.2-1.5 kb) found in human liver.
    • This finding expands our understanding of Factor H heterogeneity and its potential regulatory mechanisms.