Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Colonic mucosectomy using laser photodynamic therapy.

D G Fisher1, E B Rypins, L R Watson

  • 1Department of Surgery, University of California, Irvine Medical Center, Orange 92668.

The Journal of Surgical Research
|June 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Language Gap: Disparities in Expressive Versus Receptive Language in Young Children With Neurodevelopmental Conditions.

Journal of autism and developmental disorders·2026
Same author

From FOS fusions to somatic mutations in the MAPK pathway, heterogeneous genetic abnormalities cause distinct pathophysiology among subsets of epithelioid haemangiomas.

The British journal of dermatology·2022
Same author

Accuracy of Intercepting Moving Tactile Targets.

Perception·2019
Same author

Marinesco bodies and substantia nigra neuron density in Parkinson's disease.

Neuropathology and applied neurobiology·2017
Same author

Coexistence of Eph receptor B1 and ephrin B2 in port-wine stain endothelial progenitor cells contributes to clinicopathological vasculature dilatation.

The British journal of dermatology·2017
Same author

Ultrastructural characterization of hyperactive endothelial cells, pericytes and fibroblasts in hypertrophic and nodular port-wine stain lesions.

The British journal of dermatology·2017

Photodynamic therapy (PDT) precisely controls colonic tissue injury by varying light energy density. This study demonstrates PDT

Area of Science:

  • Biomedical Engineering
  • Gastroenterology
  • Oncology

Background:

  • Photodynamic therapy (PDT) utilizes photosensitizing agents and light to induce targeted tissue necrosis.
  • Precise control over PDT-induced injury depth is crucial for therapeutic applications, particularly in sensitive tissues like the colon.

Purpose of the Study:

  • To investigate the dose-dependent sensitivity of colonic tissue to photodynamic therapy.
  • To establish a quantitative relationship between light energy density and the depth of photodynamic injury in the colon.

Main Methods:

  • Rats received an intraperitoneal injection of Photofrin II, a photosensitizer.
  • A 1.0-cm² mucosal area of the left colon was exposed to 630 nm red light at varying energy densities (10-80 J/cm²).
  • Injury depth was measured histologically and correlated with applied energy density using nonlinear regression analysis.

Related Experiment Videos

Main Results:

  • A biphasic relationship was observed between energy density and injury depth, fitting the equation Y = 1 - ae^(bx).
  • Colonic mucosa demonstrated higher sensitivity to PDT compared to the underlying muscularis layer.
  • Extrapolation predicted a risk of bowel perforation at energy densities of 100 J/cm² and above.

Conclusions:

  • Photodynamic therapy offers a controllable method for inducing injury in colonic tissue.
  • The differential sensitivity of colonic layers suggests a safety margin against perforation during PDT.
  • Photodynamic colonic mucosectomy is a feasible therapeutic approach based on these findings.