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Nuclease-functionalized poly(styrene-b-isobutylene-b-styrene) surface with anti-infection and tissue integration
Shuaishuai Yuan1, Jie Zhao, Shifang Luan
1State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, People's Republic of China.
ACS Applied Materials & Interfaces
|September 26, 2014
Summary
This study functionalized poly(styrene-block-isobutylene-block-styrene) (SIBS) biomaterials with nucleases to prevent bacterial adhesion and biofilm formation. The modified SIBS effectively inhibited bacterial growth without negatively impacting tissue cell adhesion.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Infectious Disease Research
Background:
- Hydrophobic thermoplastic elastomers like poly(styrene-block-isobutylene-block-styrene) (SIBS) are used in biomedical applications.
- Bacterial contamination and infection pose significant challenges to biomaterial performance and patient safety.
- A key challenge is inhibiting bacterial colonization while maintaining favorable interactions with host cells.
Purpose of the Study:
- To develop a method for functionalizing SIBS substrates with nucleases to create antibacterial surfaces.
- To evaluate the efficacy of nuclease-modified SIBS in preventing bacterial adhesion and biofilm formation.
- To assess the biocompatibility of the nuclease-modified SIBS with tissue cells.
Main Methods:
- SIBS substrates were modified with nucleases using polycarboxylate grafts as an intermediate under mild conditions.
- Bacterial adhesion and biofilm formation assays were performed on modified and unmodified SIBS.
- Cell adhesion assays using L929 cells were conducted to evaluate biocompatibility.
Main Results:
- Nuclease-functionalized SIBS effectively prevented bacterial adhesion and biofilm formation.
- The nuclease coatings did not adversely affect the adhesion of L929 cells compared to virgin SIBS.
- The modification process was achieved under mild conditions.
Conclusions:
- Nuclease-modified SIBS demonstrates potent antibacterial properties, inhibiting bacterial adhesion and biofilm formation.
- The developed nuclease coating strategy preserves or enhances tissue-cell interactions, crucial for biomedical applications.
- This approach offers a promising strategy for developing infection-resistant polymeric biomaterials.
