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In vitro evaluation of antimicrobial activity of different Gutta-percha points and calcium hydroxide pastes
Ashu Jhamb1, Vishwajit Rampratap Chaurasia2, Vinay Kumar S Masamatti2
1Department of Conservative Dentistry and Endodontics, Yamuna Institute of Dental Sciences and Research, Gadholi, Yamunanagar, Haryana, India.
Purpose:
To evaluate the antimicrobial activity of different compositions of Gutta-percha points and calcium hydroxide (Ca(OH)2) pastes, used in endodontic therapy.
Materials And Methods:
The evaluated material consisted of Gutta-percha points containing Ca(OH)2, Gutta-percha points containing chlorhexidine (Chx), conventional Gutta-percha points and Ca(OH)2 pastes. Antimicrobial properties of Chx and CaOH paste are compared with CaOH points. Antimicrobial tests included three species of microorganisms: Escherichia coli (ATCC 25923), Staphylococcus aureus (ATCC 25922) Pseudomonas aeruginosa (ATCC BAA-427), the agar disc diffusion method was employed. The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37°C for 24 h. Zones of inhibition were measured.
Results And Conclusion:
All microbial species used in the study were inhibited by the Gutta-percha points containing Chx and by the Ca(OH)2 pastes, no antimicrobial activity was observed for the other groups (conventional Gutta-percha and Ca(OH)2 group).
