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Updated: Apr 23, 2026

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
Published on: July 17, 2019
Doxycycline enhances survival and self-renewal of human pluripotent stem cells
Mi-Yoon Chang1, Yong-Hee Rhee2, Sang-Hoon Yi1
1Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea.
Abstract:
We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These doxycycline effects are not associated with its antibacterial action, but mediated by direct activation of a PI3K-AKT intracellular signal. These findings indicate doxycycline as a useful supplement for stem cell cultures, facilitating their growth and maintenance.
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