Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

Biophysical journal·2026

Same author

Cancers modulate p53 truncal neoantigen display to evade T cell detection.

bioRxiv : the preprint server for biology·2025

Same author

Atomistic TCR-ligand interactions instruct memory T-cell differentiation.

bioRxiv : the preprint server for biology·2025

Same author

Single-molecule imaging prefusion intermediate conformations of MERS-CoV spike trimers in membrane during entry.

Cell reports·2025

Same author

Load-based divergence in the dynamic allostery of two TCRs recognizing the same pMHC.

eLife·2025

Same author

Biophysical and Structural Features of αβT-Cell Receptor Mechanosensing: A Paradigmatic Shift in Understanding T-Cell Activation.

Immunological reviews·2025

Same journal

Molecular crowding and amyloidogenic self-assembly: Emergent perspectives from modern computations.

Current opinion in structural biology·2026

Same journal

Recent advances in modeling and simulation of biological phenomena in crowded and cellular environments.

Current opinion in structural biology·2026

Same journal

Progress toward linking single-molecule behavior and condensate material properties.

Current opinion in structural biology·2026

Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026

Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026

Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 23, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Combining single-molecule manipulation and single-molecule detection.

Juan Carlos Cordova¹, Dibyendu Kumar Das¹, Harris W Manning¹

¹Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United States.

Current Opinion in Structural Biology

|September 26, 2014

Summary

This summary is machine-generated.

Single-molecule force microscopy and fluorescence detection reveal biomolecular machinery mechanisms. This review covers force-fluorescence microscopy, optical traps, magnetic traps, and atomic force microscopy for advanced molecular insights.

More Related Videos

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Related Experiment Videos

Last Updated: Apr 23, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Area of Science:

Biophysics
Molecular Biology
Biotechnology

Background:

Single-molecule manipulation and fluorescence techniques are crucial for understanding biomolecular machinery.
Combining these methods offers enhanced mechanistic insights.

Purpose of the Study:

To review force-fluorescence microscopy as a powerful technique.
To highlight advancements in combining manipulation and detection methods.
To discuss future potential in molecular machinery studies.

Main Methods:

Review of force-fluorescence microscopy.
Discussion of optical traps, magnetic traps, and atomic force microscopy.
Overview of fluorescence-based force sensors for in vivo measurements.

Main Results:

Force-fluorescence microscopy integrates manipulation and detection effectively.
Significant milestones achieved in combining these techniques.
Emerging strategies like in vivo force sensing show promise.

Conclusions:

Combined single-molecule manipulation and detection provide unprecedented views of molecular machinery.
The field is rapidly advancing with exciting technological demonstrations.
This integrated approach is poised to revolutionize molecular studies.