S100A6 is secreted from Wharton's jelly mesenchymal stem cells and interacts with integrin β1
Ewelina Jurewicz1, Agnieszka Góral1, Anna Filipek1
1Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
Abstract:
S100A6 is a calcium binding protein belonging to the S100 family. In this work we examined the function of extracellular S100A6. Using mesenchymal stem cells isolated from Wharton's jelly of the umbilical cord (WJMS cells) we have shown that S100A6 is secreted by these cells, and when added to the medium, increases their adhesion and inhibits proliferation. The search for a potential target/receptor of S100A6 in the membrane fraction of WJMS cells allowed us to identify some proteins, among them integrin β1, which interacts with S100A6 in a calcium dependent manner. The interaction between S100A6 and integrin β1, was then confirmed by ELISA using purified proteins. Applying specific antibodies against integrin β1 reversed the effect on cell adhesion and proliferation observed in the presence of S100A6 which indicates that S100A6 exerts its function due to interaction with integrin β1. Since the data show the influence of extracellular S100A6 on cells isolated from Wharton's jelly, our results might help to establish molecular mechanisms leading to some pathologies characteristic for this tissue.
More Related Videos
08:55Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
Published on: July 6, 2022
06:27A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
Published on: August 8, 2019
Related Concept Videos
Intracellular Signaling Affects Focal Adhesions
Some...
Overview of Cell-Matrix Interactions
Activation of Integrins
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
Integrins
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Anchoring Junctions
Mesenchymal Stem Cells
