Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

7.5K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.5K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

15.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
15.9K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.1K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.1K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.7K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.0K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell-based screen identifies translation state modulators that extend lifespan in D. melanogaster and C. elegans.

The journals of gerontology. Series A, Biological sciences and medical sciences·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026
Same author

Defining Microbiome Impact on Host Physiology During Spaceflight Using Caenorhabditis elegans.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

<i>Caenorhabditis</i> Intervention Testing Program: the anticonvulsant levetiracetam does not extend lifespan in nematodes.

microPublication biology·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026
Same author

Seeing through fibers: unsupervised image reconstruction in fiber bundle imaging systems.

Optics express·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026
Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: Apr 23, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.0K

Flow-scanning optical tomography.

Nicolas C Pégard1, Marton L Toth, Monica Driscoll

  • 1Princeton University, Department of Electrical Engineering, Olden Street, Princeton, NJ 08544, USA. jasonf@princeton.edu.

Lab on a Chip
|September 27, 2014
PubMed
Summary
This summary is machine-generated.

We developed a non-invasive 3D tomography technique for observing microscopic samples in vivo. This method captures multiple angles simultaneously, enabling detailed 3D absorption and phase imaging of live organisms like C. elegans nematodes.

More Related Videos

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.0K
Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
09:56

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Published on: August 21, 2019

6.4K

Related Experiment Videos

Last Updated: Apr 23, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.0K
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.0K
Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
09:56

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Published on: August 21, 2019

6.4K

Area of Science:

  • Biophysics
  • Microscopy
  • Optical Imaging

Background:

  • In vivo microscopic imaging is crucial for understanding biological processes.
  • Existing techniques often struggle with sample motion and limited 3D information.
  • Non-invasive methods are preferred for observing live organisms.

Purpose of the Study:

  • To present a novel 3D tomography technique for in vivo microscopic observation.
  • To enable simultaneous acquisition of multiple perspective angles for enhanced imaging.
  • To demonstrate the technique's capability for both absorption and phase contrast imaging.

Main Methods:

  • Combines microfluidic flow, slit aperture illumination, and Fourier lens for multi-angle acquisition.
  • Utilizes a limited-domain inverse Radon transform for 3D absorption retrieval.
  • Employs computational refocusing and complementary angle comparison for 3D differential phase contrast imaging.

Main Results:

  • The technique is non-invasive and robust to sample motion.
  • Successfully implemented on a modified glass slide compatible with standard microscopes.
  • Demonstrated simultaneous 3D amplitude and phase tomography on live, freely swimming C. elegans nematodes.

Conclusions:

  • The presented 3D tomography technique offers a powerful new tool for in vivo microscopic imaging.
  • It provides simultaneous absorption and phase contrast information with high robustness.
  • This method advances the study of live biological samples at the microscopic level.