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[Effect of tumor necrosis factor receptor associated factor 6 gene silencing by small interfering RNA on

Xia Li1, Mingzhen Xiao, Qing Yu

  • 1Department of Operative Dentistry and Endodontics, Hospital of Stomatology, Shanxi Medical University, Taiyuan 030001, China.

Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology
|September 27, 2014
PubMed
Abstract

Insights

Inhibiting tumor necrosis factor receptor associated factor 6 (TRAF6) gene with siRNA in MDPC-23 cells significantly decreased TRAF6 expression. This inhibition enhanced MDPC-23 cell proliferation, suggesting a role in dentin formation and repair.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Odontoblast-like cells (MDPC-23) play a crucial role in dentin formation and repair.
  • Tumor necrosis factor receptor associated factor 6 (TRAF6) is a signaling molecule implicated in various cellular processes.
  • Understanding the role of TRAF6 in odontoblast function is essential for regenerative dentistry.

Purpose of the Study:

  • To investigate the effect of inhibiting the TRAF6 gene using small interfering RNA (siRNA) in MDPC-23 cells.
  • To determine the impact of TRAF6 inhibition on MDPC-23 cell proliferation.

Main Methods:

  • Construction and delivery of siRNA vectors targeting TRAF6 into MDPC-23 cells.
  • Stable cell line establishment via G418 screening and colony culture.
  • Quantitative assessment of TRAF6 gene and protein expression using RT-PCR and Western blotting.
  • Evaluation of cell proliferation using MTT and flow cytometry assays.

Main Results:

  • Successful inhibition of TRAF6 mRNA and protein expression in MDPC-23 cells transfected with pSUPER-TRAF6siRNA compared to controls.
  • Significant increase in MDPC-23 cell proliferation, indicated by MTT assay (A value) and proliferation index (PrI), in the TRAF6-inhibited group.

Conclusions:

  • siRNA-mediated inhibition of TRAF6 effectively reduces its transcription and expression in MDPC-23 cells.
  • Inhibition of TRAF6 enhances the proliferation of MDPC-23 cells.
  • TRAF6 may play a regulatory role in dentin formation, repair, tooth eruption, and post-injury dentin regeneration.

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